The Effect of Interval Training on PPARy-GLUT4 Expression in Subcutaneous Adipose Tissue of Male Wistar Obese Rats

Introduction: Genetic and metabolic dysfunctions are primary contributors to the onset and intensity of type 2 diabetes (T2D) through the promotion of insulin resistance. The objective of this research was to examine the impact of high-intensity interval training (HIIT) on the expression levels of PPARγ and GLUT4 in the subcutaneous adipose tissue, as well as its influence on insulin sensitivity among obese rats. Methods: Obesity was induced by high fat diet (HDF) in 14 male wistar rats.  Then rats were divided randomly into exercise (HIIT, n = 7) or control (n = 7) groups. The exercise group completed an 8 weeks HIIT (5 days / weekly) and the control group received no training. Fasting blood glucose, insulin sensitivity and PPARγ and GLUT4 gene expression in subcutaneous adipose tissue were measured 48 hours after last exercise session. Independent t-test was used to compare variables between groups. Results: HIIT resulted in a significant decrease in fasting blood glucose (P = 0.001) and increase in insulin sensitivity (P = 0.001), PPARγ expression (P = 0.038) and GLUT4 expression (P = 0.019) in subcutaneous adipose tissue compared with control rats. Conclusion: HIIT can be improve insulin sensitivity in obese rats. This improvement may be attributed to increased PPARγ and GLUT4 expression in subcutaneous adipose tissue.