Temperature-Induced Precipitation of Specific DNA Fragments Using DNA-Poly(Nisopropylacrylamide) Conjugate

Soluble conjugates between double stranded DNA and poly (N-isopropylacrylamide) (polyNIPAAm) was synthesized and applied to the selective separation of DNA fragments: the target fragments were connected to the DNA-polyNIPAAm conjugate with the aid of T4 DNA ligase, followed by temperature-induced precipitation. Target DNA was collected from the resultant precipitate in high purity.