Electrochemical Evaluation of Interaction between Avidin and Biotin Self-assembled Using Marker Ions

The interaction between avidin and biotin as self-assembled monolayer on a gold electrode surface was electrochemically evaluated by monitoring the changes in the electrode response of the ferrocyanide ion as redox-marker. It was clarified that the permeability changes of avidin membrane introduced onto the biotin self-assembled monolayer against ferrocyanide ion were ascribed to electrostatic interaction between avidin and marker ion.