Gene Detection Based on the Tetrakis-acridinyl Peptide (TAP) Cassette

The TAP cassette, formed by the reaction of TAP with a DNA probe carrying a continuous AT sequence, was used for the detection of target DNA by the sandwich assay on the titer plate. This was achieved by the characteristics of TAP having high affinity and high preference for double stranded DNA and dramatic enhancement of fluorescence of TAP bound to [poly(dA-dT)]2.