Colloidal Nanoparticles from Poly(N-isopropylacrylamide)-graft-DNA for Single Nucleotide Discrimination Based on Salt-induced Aggregation: Extension to Long Target DNA

The DNA-linked colloidal nanoparticle was prepared through the phase transition of the amphiphilic copolymer consisting of DNA as the hydrophilic part and thermo-responsive poly(N-isopropylacrylamide) as the hydrophobic part. We have succeeded in detecting the single nucleotide difference of 39-mer target DNA by using a salt-induced aggregation of the particles.