Author/Authors :
Mathew، John S. نويسنده , , Saliki، Jeremiah T. نويسنده , , Ewing، Sidney A. نويسنده , , Lehenbauer، Terry W. نويسنده , , Panciera، Roger J. نويسنده , , Malayer، Jerry R. نويسنده , , Cummings، Connie A. نويسنده , , Kocan، A. Alan نويسنده ,
Abstract :
American canine hepatozoonosis (ACH), caused by Hepatozoon americanum, is an emerging tick-borne disease of dogs. An indirect enzyme-linked immunosorbent assay (ELISA) that should facilitate diagnosis of infection and study of the epidemiology of ACH has been developed using H americanum sporozoites as antigen. Efficacy of the new test as a diagnostic tool was compared with that of skeletal muscle biopsy, the current gold standard for confirming H. americanum infection. Results show that the test is sensitive (93%) and specific (96%) and that it is as reliable as histopathologic examination of skeletal muscle for detecting infection. The ELISA would be suitable as a routine laboratory test for diagnosis of ACH.
