MITOCHONDRIA-BASED AMPEROMETRIC BIOSENSOR FOR THE DETERMINATION OF L-SUCCINIC ACID

A mitochondria based amperometric biosensor was developed for the determination of Lsuccinic acid. The biosensor was characterized using hexacyanoferrate (III) as the electrochemical mediator. The electrochemical behaviour of the redox couple hexacyanoferrate (II)/(III) was improved by incorporating octadecylamine into the enzyme modified carbon paste. L-succinic acid was detected at + 0.35 V. Linearity was observed in the range 0.05–0.4 mM of L-succinic acid (sensitivity 0.388 uA/mM, r = 0.996). The detection limit is 0.02 mM. The electrode maintains 60% of its activity after 7 days of use.