CHIRAL RESOLUTION OF TWO POTENTIAL AROMATASE INHIBITORS ON CELLULOSE TRIS (4-METHYL BENZOATE) AND CELLULOSE TRIS (3,5DIMETHYLPHENYL CARBAMATE) CHIRAL STATIONARY PHASES

The chiral resolution of 1-[(benzofuran-2-yl)-5-nitrophenylmethyl] tetrazole (I) and 1[(benzofuran-2-yl)-5-bromophenylmethyl] tetrazole (II), the novel aromatase inhibitors, has been achieved on Chiracel OD, Chiracel OD-RH columns which are coated with cellulose [tris (3,5-dimethylphenyl carbamate)]and Chiracel OJ-R column coated with cellulose [tris(4methyl benzoate)]. The mobile phases used were hexane-2-propanol (95:5) on Chiracel OD and water-acetonitrile(45:55) on Chiracel OD-RH and Chiracel OJ-R columns respectively. Compound I has been resolved on Chiracel OD(a = 1.30 and Rs = 3.13) and OD-RH (a = 1.15 and Rs = 1.20)columns while compound II has been resolved on OJ-R(a = 1.31 and Rs = 1.80) column only. The effect of acetonitrile concentration in water-acetonitrile mobile phase was studied on the retention and resolution of both compounds.