A SIMPLE METHOD FOR CHIRAL SEPARATION OF EPHEDRINES USING (R)-1-NAPHTHYLGLYCINE AND 3,5-DINITROBENZOIC ACID AS STATIONARY PHASE

High performance liquid chromatography (HPLC) method using Pirkle-type chiral stationary phase (CSP) was developed for the separation of the enantiomers of ephedrine, norephedrine and pseudoephedrine, which were central nervous stimulants. The resolutions were made using normal-phase methodology with a mobile phase consisting of n-hexane, 1,2dichloroethane and methanol. Conditions for the experiments were optimized, including the content of methanol, 1,2-dichloroethane and acetic acid in mobile phase, and the effect of temperature on separation was also investigated.