Title of article
Partitioning of a Bacillus alkaline protease in aqueous two-phase systems
Author/Authors
Rashmi Sinha، نويسنده , , S. P. Singh، نويسنده , , S. Ahmed، نويسنده , , S. K. Garg، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 1996
Pages
4
From page
163
To page
166
Abstract
The partitioning behaviour of an alkaline protease from a Bacillus sp. was studied in aqueous two-phase systems comprising polyethylene glycol (PEG-6000) and K2HPO4. Based on the binodal curve, various combinations of PEG-6000 and K2HPO4 were employed for enzyme partitioning and one with 5% (w/v) PEG-6000 and 35% (w/v) K2HPO4 was found most effective. The enzyme activity was substantially stimulated by PEG-400, PEG-6000 and K2HPO4.
Keywords
Alkaline protease , Bacillus sp. , aqueoustwo-phase system , enzyme partitioning , enzyme activation.
Journal title
Bioresource Technology
Serial Year
1996
Journal title
Bioresource Technology
Record number
410093
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