Production of biomass and nutraceutical compounds by Spirulina platensis under different temperature and nitrogen regimes

The cyanobacterium Spirulina platensis has been used by humans because of its nutritional and possibly medicinal effects. Our study evaluated the influence of temperature and nitrogen concentration in the medium on the production of biomass by this cyanobacterium and the biomass composition in protein, lipid and phenolic compounds. We found that at 35 °C there was a negative effect on biomass production but a positive effect on the production of protein, lipids and phenolics, the highest levels of these compounds being obtained in Zarrouk’s medium containing 1.875 or 2.500 g l−1 sodium nitrate. Higher biomass densities and productivity were obtained at 30 °C than at 35 °C, but nitrogen concentration appeared to have no effect on the amount of protein, lipid or phenolics, indicating that at 30 °C the concentration of sodium nitrate in Zarrouk’s medium (2.50 g l−1) can be reduced without loss of productivity, an important cost-saving factor in large-scale cultivation.