Abstract :
In order to get more detailed irtfortnation on single proteins
involved in the life cycle of Abutilon mosaic geminivirus,
special conditions were established allowing
expression of the proteins encoded by different viral open
reading frames (AC2, AC3, AVI, BC1, and BV1) in Escherichia
coli by use of the pET expression vector system.
These include cotransformation of a tRNA supplementation
plasmid to overcome codon usage problems
in the case of AC2, Protocols were developed to purify
the virus proteins for production of antisera. Phosphorylation
of AbMV proteins AC3, AVI, BCl, and
BV 1 in £. coli could be shown by autoradiographic detection
of stably incorporated inorganic ʹ-PO4".
