RAPD and SCAR Markers Linked to a Gene Conferring Resistance to Angular Leaf Spot in Common Bean

Angular leaf spot\ caused by the fungus Phaeoisariopsis `riseola\ is one of the most important bean diseases in Brazil[ The objectives of this study were to determine the inheritance of angular leaf spot resistance and to identify random ampli_ed polymorphic DNA "RAPD# and sequence!characterized ampli_ed region "SCAR# mar! kers linked to the resistance gene present in cv[ Cornell 38 131\ in a cross between this cultivar and susceptible cv[ Ruda [ The parents\ F0\ F1 and backcross!derived plants were inoculated with P[ `riseola pathotype 20 06 under environmentally controlled greenhouse conditions[ The results indicate that one single dominant gene con! trols the resistance in Cornell 38 131[ Two RAPD mark! ers\ OPN 91789c and OPE 93549c\ were found to be linked in the coupling phase\ at 2[1 and 01[4 cM of the resistance gene\ respectively[ To increase the reproducibility of the detection of marker OPN91789c it was converted into a SCAR marker[ It is proposed that the designation of Ph`!1 be used for the angular leaf spot resistant gene present in Cornell 38 131[