Amplified fragment length polymorphism (AFLP) as suitable markers to study orobanche cumana genetic diversity

Orobanche cumana Wallr[\ an obligate root parasite of sun~ower can cause severe damage to this crop[ The genetic diversity obtained with random ampli_ed poly! morphic DNA "RAPD# and ampli_ed fragment length polymorphism "AFLP# on two Orobanche populations were compared[ Nei and Li distance matrices obtained with both methods among the two populations were cor! related signi_cantly according to Mantel|s test and could partition the populations[ The sampling variance of gen! etic distances within and among populations estimated using bootstrap procedure were not signi_cantly di}erent between the two techniques[ The principal di}erence between the two techniques is that AFLP markers gave a higher degree of resolution for discriminating closely related germplasm than RAPD[