Antifungal Activity of a Bowman–Birk-type Trypsin Inhibitor from Wheat Kernel

A trypsin inhibitor from wheat kernel "WTI# was found to have a strong antifungal activity against a number of pathogenic fungi and to inhibit fungal trypsin!like activity[ WTI inhibited in vitro spore germination and hyphal growth of pathogens\ with protein concentration required for 49) growth inhibition "IC49# values ranging from 000[6 to above 499 mg:ml[ As observed by electron microscopy\ WTI determined morphological alterations represented by hyphal growth inhibition and branching[ One of the fungal species tested\ Botrytis cinerea pro! duced a trypsin!like protease\ which was inhibited by the trypsin inhibitor[ WTI\ as well as other seed defence proteins\ appear to be an important resistance factor in wheat kernels during rest and early germination when plants are particularly exposed to attack by potential soil! borne pathogens[