Title of article
Production of Polyclonal Antibodies to a Recombinant Coat Protein of Potato mop-top virus
Author/Authors
N. ?e?ovsk?، نويسنده , , T. Moravec، نويسنده , , P. Roseck?، نويسنده , , P. D?di? and M. Filigarov?، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2003
Pages
6
From page
195
To page
200
Abstract
The coat protein (CP) coding regions of two Czech
Potato mop-top virus (PMTV) isolates were sequenced
and shown to be identical. One, the Korneta isolate
CP gene, was cloned in several expression vectors. The
recombinant PMTV-CP was expressed in Escherichia
coli and the purified recombinant protein was used to
produce PMTV-specific polyclonal antibodies. The
antiserum had a titre of 1 : 2000 in an indirect
enzyme-linked immunosorbent assay (ELISA) and
reacted specifically in immunoblotting and IPTAELISA
(indirect plate-trapped antigen (PTA)-ELISA).
Keywords
Potato mop-top virus , recombinant coat protein , Escherichia coli
Journal title
Journal of Phytopathology
Serial Year
2003
Journal title
Journal of Phytopathology
Record number
428311
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