Author/Authors :
E.-D. AMMAR، نويسنده , , R. G. GOMEZ-LUENGO، نويسنده , , D. T. GORDON and S. A. HOGENHOUT، نويسنده ,
Abstract :
Maize Iranian mosaic virus (MIMV) was characterized
and compared with isolates of Maize mosaic virus
(MMV, genus Nucleorhabdovirus, family Rhabdoviridae)
in insect transmission, cytopathology and ultrastructure
of infected maize cells, virion proteins and
serologically. MIMV is naturally transmitted by Ribautodelphax
notabilis, a delphacid planthopper, in Iran.
In this study, another planthopper, Peregrinus maidis,
vector of MMV, transmitted MIMV with an estimated
efficiency of 0.4–1.6% following feeding on MIMVinfected
maize plants and 64% following injection of
MIMV into the hemolymph, suggesting that P. maidis
gut tissues largely blocked MIMV transmission.
MIMV and MMV-HI (Hawaii) induced similar cytopathologies
in cells of infected maize leaves, with virions
budding through inner nuclear and endoplasmic reticulum
membranes. In thin sections, virions of MIMV
were significantly shorter than those of MMV-HI.
Sodium dodecyl sulphate polyacrylamide gel electrophoresis
analysis of virions of MIMV, MMV-HI,
MMV-CR (Costa Rica) and MMV-FL (Florida)
yielded six proteins of which four were identified as
the putative G, N, P and M proteins of plant rhabdoviruses.
The N, P and M proteins of MIMV migrated
faster in gels than those of the MMV isolates indicating
a lower molecular weight, whereas the bands corresponding
to the G proteins migrated similarly for
both viruses. Polyclonal antibodies to MMV-HI failed
to react with virions of MIMV in enzyme-linked immunosorbent
assay (ELISA) and with MIMV proteins
in Western blots. In contrast, these antibodies reacted
strongly with MMV-HI and MMV-FL virions in
ELISA and with MMV-HI, MMV-CR and MMV-FL
proteins in Western blots. Further, in ELISA, polyclonal
antibodies to MMV-MR (Mauritius) reacted
weakly with MIMV virions but strongly with
MMV-HI and MMV-FL virions. Thus, it is concluded
that MIMV is a new virus of the Nucleorhabdovirus
genus that may be distantly related to MMV
Keywords :
virion proteins , Western blots , Enzyme-linked immunosorbent assay , insect transmission , ultrastructure
