First Report of Hop stunt viroid and Citrus exocortis viroid on Fig with Symptoms of Fig Mosaic Disease

Reverse transcription polymerase chain reaction (RTPCR) was used for the detection of Hop stunt viroid (HSVd) and Citrus exocortis viroid (CEVd) in nucleic acid extracts of infected fig leaf tissues. RT-PCR results were confirmed by cDNA sequencing. This is the first report of CEVd and HSVd infecting fig in Tunisia. Alignments and phylogenetic analysis classify the HSVd Tunisian isolates in the plum-type group. CEVd Tunisian isolates are very closely related to the CEVd tomato hybrid callus isolate and to CEVd Gynura isolate from the USA.