Effect of Impaction, Bounce and Reaerosolization on the Collection Efficiency of Impingers

The collection efficiency of liquid impingers was studied experimentally as a function of the sampling flow rate with test particles in the bacterial size range. Three impingers were tested: two All-Glass Impingers (AGI-4 and AGI-30), widely used for bioaerosol sampling, and a newly developed slot impinger. The aerosol particles were generated by a Collison nebulizer, and an Aerosizer was used to measure the particle concentrations and size distributions upstream and downstream of each impinger. The effect of the air pressure drop across the impinger on the Aerosizer performance was investigated, and the particle measurement system was modified and calibrated accordingly. While inertial impaction is the dominant particle removal mechanism in impingers, particle bounce and reaerosolization were also found to have significant effects on the impinger collection characteristics. At relatively high flow rates and low levels of collection fluid (corresponding to the collection fluid level after evaporation of most of the liquid during prolonged impingement), the liquid under the impinger jet was observed to be removed by the air pressure and pushed against the containerʹs walls. Particles, such as bacterial or fungal spores, may thus bounce from the bottom of the collection vessel and escape with the eilluentair flow or may impact sideways into the liquid that was previously pushed against the waIls. It was found that such particle bounce may significantly reduce the collection efficiency of impingers containing a small amount of liquid. When the impingers were operated at a high level of collection fluid and sufficiently high sampling flow rates, it was observed that the bubbles, rising through the liquid, entrained previously collected particles and created new aerosols by bursting at the liquid-air surface. Such particle reaerosolization was also found to reduce the impinger collection efficiency