Cloning and characterization of salicylic acid-induced, intracellular pathogenesis-related gene from tomato (Lycopersicon esculentum)

Intracellular pathogenesis-related gene (IPR) from tomato was cloned from a salicylic acid (SA) induced cDNA library and was designated as TSI-1 (tomato stress induced-1). The deduced amino acid sequence of TSI-1 codes for a 178 amino acid polypeptide of molecular weight 20·4 kDa. TSI-1 is highly homologous to the potato STH-2 and STH-21 IPR and tree pollen allergens which cause type I allergic reactions in humans. TSI-1 lacks a signal peptide like other IPR members. It is organized as a multigene family and is inducible by SA and Fusarium oxysporum infection.