Antioxidant Nutrient Supplementation Reduces the Susceptibility of Low Density Lipoprotein to Oxidation in Patients With Coronary Artery Disease

Objectives. This study sought to determine the effect of antioxidant supplementation on the susceptibility of low density lipoprotein (LDL) to oxidation in patients with established cardiovascular disease (CVD). Background. Dat are inconsistent regarding the role of antioxidant nutrients in the prevention of CVD. Methods. The study design was 12-week, double-blind, placebo-controlled clinical trial. Patients with CVD (n = 45) were randomized to 1) placebo control; 2) 400 IU of vitamin E, 500 mg of vitamin C, 12 mg of beta-carotene (mid-dose); or 3) 800 IU of vitamin E, 1,000 mg of vitamin C, 24 mg of beta-carotene (high dose) daily. Reduced susceptibility of LDL to oxidation was estimated by an increase in lag phase (minutes). Baseline and 6- and 12-week measurements of lipoproteins and lag phase were obtained. Plasm levels of antioxidants were measured at baseline and 12 weeks. Results. Concentrations of alpha-tocopherol, vitamin C and beta-carotene significantly increased in the mid- and high dose groups during the trial. Lag phase significantly increased from baseline (190.1 ± 63.8 min [mean ± SD]) to 12 weeks (391.1 ± 153.0 min) in the high dose group (p < 0.01). nonsignificant increase in lag phase in the mid-dose group was observed during the same time interval. dose response was found for mean percent change from baseline to 12 weeks for lag phase for the placebo, mid- and high dose groups (p = 0.004 for trend). Conclusions. high dose combination of antioxidant nutrients reduces the susceptibility of LDL to oxidation in patients with CVD and may be useful in secondary prevention.