Peripheral blood levels of matrix metalloproteases-2 and -9 are elevated in patients with acute coronary syndromes

Objectives. This study was sought to investigate whether peripheral blood levels of matrix metalloproteases (MMPs) are affected in patients with acute coronary syndromes (ACS). Background. Synthesis of MMPs has been reported in coronary atherosclerotic lesions in patients with unstable angin (UA), suggesting pathogenic role of MMPs in the development of ACS. Methods. Using sandwich enzyme immunoassay, serum MMP-2 and plasm MMP-9 were measured in 33 patients with ACS (22 with acute myocardial infarction [AMI], 11 with UA), 17 with stable effort angin (EA) and 17 normal control subjects. Results. Serum MMP-2 in patients with U and AMI on day 0 was two times greater than that in control subjects, and patients with E showed higher MMP-2 levels than those in control subjects. Plasm MMP-9 in patients with U and AMI on day 0 was elevated by threefold and twofold versus that in control subjects, respectively. In patients with U and AMI who underwent medical treatment (n = 11 and 13, respectively), MMP-2 elevation was sustained until day 7. In patients with UA, MMP-9 elevation on day 0 was followed by gradual decrease toward the control range up to day 7. Some patients with AMI showed transient MMP-9 elevation with peak on day 3, whereas in others, MMP-9 levels were significantly elevated on day 0 and remained higher than those in control subjects up to day 3. Conclusions. Serial changes in serum MMP-2 and plasm MMP-9 were documented in patients with ACS. These findings provide an insight into the molecular mechanism of plaque destabilization.