Author/Authors :
Jean-Marc Costa، نويسنده , , Pauline Ernault، نويسنده , , Martine Olivi، نويسنده , , Thierry Gaillon، نويسنده , , Khalil Arar، نويسنده ,
Abstract :
Objective: Evaluation of the use of short probes as a detection system in real-time PCR.
Design and method: Comparison of results obtained with hybridization probes with and without locked nucleic acid (LNA) residues in the detection of fetal DNA in maternal serum.
Results: The use of chimeric LNA/DNA probes led to a slight but significantly higher level of sensitivity as well as a higher level of fluorescence signal.
Conclusion: Chimeric LNA/DNA probes offer an interesting alternative detection method in real-time PCR.
