Comparative study between the Light Cycler® and the PCR-restriction fragment length polymorphism in detecting factor V Leiden and factor II 20210G>A mutations

Objectives To test reproducibility, speed and cost of testing for factor V Leiden and FII 20210G>A in our practice. Design and methods We compared conformity, reproducibility, speed and cost using the Light Cycler (LC®) and PCR-RFLP. Results There was 100% conformity and reproducibility. LC® was faster but 23% more expensive per sample. When equipment depreciation and patient expenses are added, LC® testing becomes cheaper. Conclusion In our practice, LC® provides fast, reproducible and cost-effective results.