Author/Authors :
Christoforos Kalleas، نويسنده , , Ioannis Tentes، نويسنده , , Dimitrios Margaritis، نويسنده , , Kostas Anagnostopoulos، نويسنده , , Athina Toli، نويسنده , , Dimitrios Pendilas، نويسنده , , Georgios Bourikas، نويسنده , , Constantinos Tsatalas، نويسنده , , Alexandros H. Kortsaris، نويسنده ,
Abstract :
Objectives:
The effect of the presence of HbS in the determination of HbA2 using the Biorad Variant II analyzer.
Design and methods:
The effect of HbS presence in the samples was quantified using the HELENA SAS-MX alkaline gel electrophoresis kit as the reference method.
Results:
The %HbA2 values from the Variant II analyzer and the HELENA SAS-MX alkaline gel electrophoresis kit show a good linear correlation in the absence of HbS. A strong positive bias in the %HbA2 values from the Variant II is apparent in the presence of HbS in the samples, when compared to the alkaline electrophoresis gel.
Conclusion:
The Variant II analyzer gives reliable results for %HbA2 determination when no HbS is detectable in the samples. When HbS is present, the gel electrophoresis method gives more accurate results.
Keywords :
HPLC , Method comparison , Gel electrophoresis , Haemoglobin A2 , Haemoglobin S
