Author/Authors :
Gino Giannaccini، نويسنده , , Laura Giusti، نويسنده , , Ferruccio Santini، نويسنده , , Alessandro Marsili، نويسنده , , Laura Betti، نويسنده , , Giovanni Mascia، نويسنده , , Caterina Pelosini، نويسنده , , Stefano Baroni، نويسنده , , Federica Ciregia، نويسنده , , Laura Fabbrini، نويسنده , , Antonio Lucacchini، نويسنده , , Paolo Vitti، نويسنده , , Aldo Pinchera، نويسنده ,
Abstract :
Objectives:
The presence of tubby protein in human lymphocytes was investigated and their electrophoretic mobility property between normal weight and obese subjects was compared.
Design and methods:
2-DE proteome map of lymphocytes has been generated and western blot analysis was conducted using anti-tub polyclonal antibody.
Results and conclusions:
We found the presence of tubby protein both in normal weight and in obese subjects; however in the latter an isoelectric point shift toward the acidic end was observed.
Keywords :
obesity , two-dimensional electrophoresis , Lymphocytes , Tubby protein
