Comparison of automated assays for the determination of vitamin B12 in serum

Objectives: To study the agreement of serum cobalamin results obtained with three different widely used analyzers. Design and methods: Comparative measurement of 100 serum samples was performed using the Abbott Architect, the Bayer Centaur, and the Roche Elecsys 2010 analyzer. Results: Relatively close numeric correlation of the results was found with Pearsonʹs r > 0.95 for all three comparisons; however, the mean concentration of the highest reading and the lowest reading method differed by 16%. Conclusions: Relatively good agreement of results from three automated serum cobalamin assays was observed.