A new concept for the construction of an artificial dehydrogenase for fructosylamine compounds and its application for an amperometric fructosylamine sensor

For the construction of an artificial dehydrogenase and an artificial dehydrogenase based sensor, the potential application of polyvinylimidazole (PVI) was investigated as the catalyst for the fructosylvaline (Fru-val) oxidation, a model compound for the glycated hemoglobin. The presence of PVI catalyzed the oxidation of Fru-val in the presence of an electron acceptor. A colorimetric determination of Fru-val was possible with the detection range from 50 muM to 10 mM utilizing PVI as the catalyst. An amperometric sensor for Fru-val was constructed using carbon paste electrode immobilized PVI. Using the sensor system, Fru-val could be measured from 20 muM to 0.7 mM. These results indicated the further possible application of PVI as the catalyst for fructosylamine compounds as well as various glycated proteins including glycated hemoglobin.