PTH(1-34) suppresses appositional bone formation by cultured rat cranial osteoblasts

Parathyroid hormone (PTH) provokes cell division of osteoblasts in vitro and is anabolic in vivo when administered intermittently. We studied the effect on bone formation in vitro by rat primary calvarial osteoblasts of the short-term addition of rat PTH(1-34) (10−12, 10−9, and 10−7 mol/L) to the medium. Our aim was to determine whether intermittent PTH(1-34) would promote osteogenesis in an experimental system designed to promote appositional bone formation. Unexpectedly, whether the exposure to PTH(1-34) was for 24 h only on days 3 or 12, or 24 h on both days 3 and 12, or a pulse for 6 h every 48 h, we observed suppression of bone formation even at the lowest concentration of added PTH(1-34). Our finding that cells which are of the osteoblastic phenotype can be entirely prevented from making appositional bone by high concentrations of PTH(1-34) for 1 day in a week, and have their osteogenic capacity suppressed by lower concentrations even when the PTH(1-34) is administered in brief pulses every other day, suggests that pulses of PTH administered in vivo may not increase bone formation by already differentiated osteoblasts.