Determination of human serum albumin by chemiluminescence immunoassay with luminol using a platinum-immobilized flow-cell

A new technique for analysis by means of chemiluminescence (CL) is reported. Conventional CL methods for analysis using a homogeneous catalyst can be unsuitable for stable analysis, because the conditions of mixing of the reactant solutions greatly affects the results. Though, electrochemiluminescence (ECL) is capable of solving this problem, it requires more complicated equipment than CL. A platinum plate-immobilized flow-cell is capable of stably controlling the CL from luminol as in ECL and has the advantage of requiring no electrical equipment. Human serum albumin (HSA) concentrations ranging from 0 to 100µgml-1 in water and in dialysate are measured by means of the CL intensity enhancement of luminollabeled anti-HSA antibody caused by an antigen-antibody reaction in the presence of hydrogen peroxide using the Pt-immobilized flow-cell.