Monoclonal-based enzyme-linked immunosorbent assay and immunochromatographic rapid assay for salinomycin

Salinomycin is one of the most widely used coccidiostats in the Japanese poultry industry. A simple and reliable analytical method for this drug is required. The current determination by bioautography is not sensitive and is labor-intensive. This paper reports an enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies and the development of a rapid test kit based on immunochromatography. The ELISA was quantitative in the range 0.05¯ 25.6 ng ml-1 (from 0.005 to 2.56 ng) and showed 50% inhibition (IC50) at 0.7 ng ml-1 (equivalent to 0.07 ng). The detection limits using the ELISA were 10 ng g-1 for plasma, liver and chicken muscle. The detection limit using the kit was 50 ng g-1 for plasma and 300 ng g-1 for chicken muscle.