Molecular investigation of the stromal cell-derived factor-1 chemokine in lymphoid leukemia and lymphoma patients from Brazil

The stromal cell-derived factor-1 (SDF-1) gene contains a common polymorphism, termed SDF1-3′A, in an evolutionarily conserved segment of the 3′-untranslated region (3′-UTR). We compared SDF-1 genotypes in patients diagnosed with lymphoid leukemias and lymphomas. Since the SDF1-3′A variant deletes the MspI restriction site, PCR-restriction fragment length polymorphism (RFLP) analysis was used for identification of genotypes. We identified the heterozygous genotype (3′A/wt) in 38.8% (24/62) of lymphoma patients and in 26.2% (11/42) of lymphoid leukemias. The percentage of 3′A carriers was significantly higher in lymphomas (43.5%) than in lymphoid leukemias (26.2%; P< 0.05). Our study indicates that lymphoma patients from Brazil are more likely to carry the 3′A gene than patients with lymphoid leukemias, suggesting that this polymorphism may be a differential determinant of lymphomas and lymphoid leukemia.