Indirect determination of superoxide anion radical in the plant of red sage based on vanillin-8aminoquinoline with fluorescence

A novel spectrofluorimetric method using vanillin-8-aminoquinoline (VAQ) as fluorescent probe was developed for the determination of superoxide anion radical (O2 (square root)). The new fluorescent probe was characterized by elemental analysis and IR spectra. Under the optimum conditions of the determination, the linear calibration range and the detection limit of the developed method for superoxide anion radical were in the range (0.0–1.0)×10^-5 and 2.0×10^-8 mol l^-1, respectively. The effect of interferences was studied. The proposed method was applied to determine the generation rate of superoxide anion radical in the course of aging in red sage successfully.