Strategies for direct attachment of hapten to a polystyrene support for applications in enzymelinked immunosorbent assay (ELISA)

A new method describing direct attachment of carboxylated haptens on a polystyrene support, using 3-aminopropyltriethoxysilane (3-APTES) as a linker, is reported. The hapten coated polystyrene support showed excellent stability as a function of the buffer pH and reaction time, and was successfully used to demonstrate its application in enzyme-linked immunosorbent assay (ELISA).