Author/Authors :
Marshall، Alan G. نويسنده , , Yanofsky، Corey M. نويسنده , , Bell، Alexander W. نويسنده , , Lesimple، Souad نويسنده , , Morales، Frank نويسنده , , Lam، TuKiet T. نويسنده , , Blakney، Greg T. نويسنده , , Carrillo، Brian نويسنده , , Lekpor، Kossi نويسنده , , Boismenu، Daniel نويسنده , , Kearney، Robert E. نويسنده ,
Abstract :
In high-throughput proteomics, a promising current approach is the use of liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR-MS) of tryptic peptides from complex mixtures of proteins. To apply this method, it is necessary to account for any systematic measurement error, and it is useful to have an estimate of the random error expected in the measured masses. Here, we analyze by LC-FTICR-MS a complex mixture of peptides derived from a sample previously characterized by LC-QTOF-MS. Application of a Bayesian probability model of the data and partial knowledge of the composition of the sample suffice to estimate both the systematic and random errors in measured masses.
