The effects of a caries-removing gel (carisolv) on FM3A cell-line in vitro

Objectives: The aim of this study was to examine the cytotoxic effects of Carisolv on mouse mammary carcinoma cell line (FM3A) at different application times. Methods: The FM3A cell line obtained from the European Collection of Animal Cell Cultures was used in the cell culture assays. Exponentially growing cells were seeded in 5×105 cells/well in 5 ml of RPMI 1640 medium supplemented with 10(%) fetal calf serum and antibiotics in each well of a six-well plate. Carisolv gel was applied onto the cell culture medium for 1, 5 and 20 min and incubated for 24 h at 37 °C in a humidified atmosphere of 5(%) carbon dioxide (CO2). After 24 h incubation, the cells were collected by trypsinisation and counted with a hemocytometer. The cytotoxicity of the Carisolv was determined by evaluation of cell growth and viability in comparison to untreated controls (cell GROWTH=100%). For cell viability, the trypane blue exclusion assay was used. Dunnettʹs t-test was used for statistical analysis. Results: Cell growth was significantly reduced after 20 min application of Carisolv in comparison to the control (p<0.001) and 1 min treatment groups (p<0.05). No significant differences were found in cell viability between the study groups. Conclusions: It can be concluded that prolonged application of Carisolv did not affect cell viability, but had a reducing effect on cell growth in the FM3A cell line.