Title of article
Monolithic Silica-Based Capillary Reversed-Phase Liquid Chromatography/Electrospray Mass Spectrometry for Plant Metabolomics
Author/Authors
Tanaka، Nobuo نويسنده , , Nakanishi، Kazuki نويسنده , , Tolstikov، Vladimir V. نويسنده , , Lommen، Arjen نويسنده , , Fiehn، Oliver نويسنده ,
Issue Information
دوهفته نامه با شماره پیاپی سال 2003
Pages
-6736
From page
6737
To page
0
Abstract
Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected using a 90-cm capillary column for LC separation and a noise reduction and automatic peak alignment software, which outperformed manual inspection or commercially available mass spectral deconvolution software.
Keywords
Fragaria , strawberry crinkle rhabdovirus , strawberry mottle disease , electron microscopy
Journal title
Analytical Chemistry
Serial Year
2003
Journal title
Analytical Chemistry
Record number
50735
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