Title of article :
Electric Manipulation of Bioparticles and Macromolecules on Microfabricated Electrodes
Author/Authors :
Huang، Ying نويسنده , , Ewalt، Karla L. نويسنده , , Tirado، Marcus نويسنده , , Haigis، Robert نويسنده , , Forster، Anita نويسنده , , Ackley، Donald نويسنده , , Heller، Michael J. نويسنده , , OConnell، James P. نويسنده , , Krihak، Michael نويسنده ,
Issue Information :
دوهفته نامه با شماره پیاپی سال 2001
Pages :
-1548
From page :
1549
To page :
0
Abstract :
Bioparticle separation, bioparticle enrichment, and electric field-mediated immune detection were carried out on microfabricated semiconductor chips utilizing ac and dc electric fields. Microscale separation on a chip surface having an active area of ~16 mm^2 was demonstrated for a mixture of Bacillus globigii spores and Escherichia coli bacteria. Dielectrophoretic enrichment was performed by collecting target bioparticles from a flow stream in flow cells of ~7.5 (mu)L, achieving a 20-fold increase in the concentration of E. coli bacteria from a diluted sample, a 28-fold enrichment for peripheral blood mononuclear cells from red blood cells, and a 30-fold increase in white blood cells from diluted whole blood. The ability to manipulate and collect bioparticles and macromolecules at microfabricated electrodes with ac and dc fields was further illustrated in electric field-mediated immunoassays for analyzing the biological identities of E. coli bacteria and B. globigii spores. According to these results, the electric methods for manipulating bioparticles present themselves as viable techniques for novel biomedical applications in sample preparations and biochemical assays on microelectrode arrays.
Journal title :
Analytical Chemistry
Serial Year :
2001
Journal title :
Analytical Chemistry
Record number :
50816
Link To Document :
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