From PCR to RCA: a surgical traineeʹs guide to the techniques of genetic amplification

With the advent of evidence-based medicine and the Calman–Hine Report, more and more surgical trainees are undertaking a period of research, either before entering or during their Specialist Registrar training. Many will encounter concepts in science uncommon in daily clinical settings. This paper will elucidate the techniques of genetic amplification available today with their potential for usage in clinical research.