Title of article :
Correlation between copy number of mitochondrial DNA and clinico-pathologic parameters of hepatocellular carcinoma
Author/Authors :
S. Yamada، نويسنده , , S. Nomoto، نويسنده , , T. Fujii، نويسنده , , T. Kaneko، نويسنده , , S. TAKEDA, H، نويسنده , , S. Inoue and H. Akagi، نويسنده , , N. Kanazumi، نويسنده , , A. Nakao، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
Abstract :
Aims
In the current study, we investigated possible correlations of the mtDNA copy number in hepatocellular carcinoma (HCC) with the pathological findings and prognosis.
Methods
We studied 31 HCC specimens using quantitative real-time polymerase chain reaction analysis, and the correlation between the mtDNA copy number and the clinicopathologic parameters and mutations in the D-loop region of the mitochondrial genome.
Results
The mtDNA copy number was reduced in HCCs compared with the corresponding non-cancerous liver tissues (p=0.002), and significantly correlated with tumour size (p=0.014) and cirrhosis (p=0.048). Patients with a low mtDNA copy number tended to show shorter 5-year survival rates than patients with a high mtDNA copy number when assessed by Kaplan–Meier curves, but not a significant (overall survival rate, 63 vs 83%; p=0.19). The copy number of HCC with mtDNA D-loop mutation or deletion was lower, but not significantly so (p=0.656, p=0.590, respectively).
Conclusions
Our results indicated that a reduced copy number of mtDNA is correlated with HCC and associated with malignant potential.
Keywords :
hepatocellular carcinoma , mitochondrial DNA , copy number , prognostic factor , Quantitative PCR
Journal title :
European Journal of Surgical Oncology
Journal title :
European Journal of Surgical Oncology