Title of article :
Human placenta-derived mesenchymal progenitor cells support culture expansion of long-term culture-initiating cells from cord blood CD34+ cells
Author/Authors :
Yi Zhang، نويسنده , , Changdong Li، نويسنده , , Xiaoxia Jiang، نويسنده , , Shuangxi Zhang، نويسنده , , Ying Wu، نويسنده , , Bing Liu، نويسنده , , Peihsien Tang، نويسنده , , Ning Mao، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
8
From page :
657
To page :
664
Abstract :
Objective Allogeneic transplantation with umbilical cord blood (UCB) in adult recipients is limited mainly by a low CD34+ cell dose. To overcome this shortcoming, human placenta as a novel source of human mesenchymal progenitor cell (MPC) was incorporated in an attempt to expand CD34+ cells from UCB in vitro. Materials and methods Human placenta MPC was isolated and characterized by morphologic, immunophenotypical, and functional analysis. UCB CD34+ cells were expanded by coculture with placental MPC. Suitable aliquots of cells were used to monitor cell production, clonogenic activity, and long-term culture-initiating culture (LTC-IC) output. Finally, the immunoregulatory effect of placental MPC was evaluated by T-cell proliferation assay. Results In its undifferentiated state, placental MPC displayed fibroblastoid morphology; was CD73, CD105, CD29, CD44, HLA-ABC, and CD166 positive; produced fibronectin, laminin, and vimentin; but was negative for CD14, CD31, CD34, CD45, HLA-DR, and α-smooth muscle actin. Functionally, it could be induced into adipocytes, osteocytes, and chondrocytes. In vitro expansion of UCB hematopoietic cells, when cocultured with placental MPC in the presence of cytokines, was significantly enhanced: CD34+ cells by 14.89 ± 2.32 fold; colony-forming cell (CFC) by 36.73 ± 5.79 fold; and LTC-IC by 7.43 ± 2.66 fold. Moreover, placental MPC could suppress T-cell proliferation induced by cellular stimuli. Conclusion These results strongly suggest that human placental MPC may be a suitable feeder layer for expansion of hematopoietic progenitors from UCB in vitro.
Journal title :
Experimental Hematology
Serial Year :
2004
Journal title :
Experimental Hematology
Record number :
514037
Link To Document :
بازگشت