Title of article :
DNA methylation by tert-butyl hydroperoxide-iron (II)
Author/Authors :
Sonia Hix، نويسنده , , Maur?cio Da Silva Morais، نويسنده , , Ohara Augusto، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1995
Pages :
9
From page :
293
To page :
301
Abstract :
Reduction of tert-butyl hydroperoxide (TBHP) by iron (II) at pH 4.0 or pH 7.0 in the presence of calf thymus DNA led to generation of high yields of methyl radicals and to DNA methylation. Methyl radicals were identified by spin-trapping experiments with 5,5-dimethyl-l-pyrroline N-oxide (DMPO) and α-(4-pyridyl-1-oxide)-N-tert-butylnitrone (POBN). The methylated DNA-base adducts were identified in treated DNA hydrolysates by high pressure liquid chromatography (HPLC) and photodiode array UV spectroscopy. In the case of DNA several methylated adducts were identified, namely N7-methylguanine, C8-methylguanine, N3-methyladenine, and O6-methylguanine. By contrast, 2′-deoxyguanosine is alkylated almost exclusively to C8-methyl-2′-deoxyguanosine. These results constitute the first evidence that TBHP is able to alkylate DNA.
Keywords :
Tert-butyl hydroperoxide , Hydroperoxides , free radicals , Spin-trapping , DNA methylation , Iron , DNA alkylation , DNA-adducts , C8-methylguanine , N7-methylguanine
Journal title :
Free Radical Biology and Medicine
Serial Year :
1995
Journal title :
Free Radical Biology and Medicine
Record number :
517153
Link To Document :
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