Title of article :
Cyclic amp-induced differentiation increases the synthesis of extracellular superoxide dismutase in rat c6 glioma
Author/Authors :
Stefan Nicolai، نويسنده , , Jean Willems، نويسنده , , An Zwijsen، نويسنده , , Els Van Mechelen، نويسنده , , Herman Slegers، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1996
Pages :
6
From page :
481
To page :
486
Abstract :
The effect of membrane permeable cAMP analogues on the expression of extracellular superoxide dismutase (EC-SOD) was studied in rat C6 glioma. EC-SOD is constitutively expressed but stimulation with cAMP analogues still increased the EC-SOD transcription and the secreted SOD activity. The potency to enhance EC-SOD expression is correlated with the ability of the cAMP analogue to induce cAMP-dependent differentiation in C6. The increase in EC-SOD mRNA and in secreted activity depended on the concentration of the cAMP analogues and on the cultivation time. Twenty-four hours after addition of 0.5 mM N6, Oʹ2-dibutyryl cAMP (dbcAMP) or N6monobutyryl cAMP (N6-mbcAMP) EC-SOD mRNA expression increased approximately twofold, while stimulation for 68 h with 0.5 mM N6-mbcAMP or 1 mM 8-Chloro cAMP (CIcAMP) and 1 mM dbcAMP enhanced the mean secreted activity/cell three- and fivefold, respectively. Oʹ2-monobutyryl cAMP (Oʹz-mbcAMP) did not affect ECSOD synthesis. The enhancement in EC-SOD activity did not require activation of protein kinase A. ATP, TGF-ß, IFN-y, and LPS did not affect EC-SOD synthesis. The presented data point to a cAMP-dependent pathway for the enhanced expression of EC-SOD by glial cells in brain.
Keywords :
free radicals , extracellular superoxide dismutase , cAMP analogues , differentiation , GFAP , Transcriptional regulation , Glioma
Journal title :
Free Radical Biology and Medicine
Serial Year :
1996
Journal title :
Free Radical Biology and Medicine
Record number :
517395
Link To Document :
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