Author/Authors :
Tomasz H. Zastawny، نويسنده , , Magdalena Dabrowska، نويسنده , , Tomasz Jaskolski، نويسنده , , Maciej Klimarczyk، نويسنده , , Lubomir Kulinski، نويسنده , , Agnieszka Koszela، نويسنده , , Maciej Szczesniewicz، نويسنده , , Magdalena Sliwinska، نويسنده , , Piotr Witkowski، نويسنده , , Ryszard Olinski، نويسنده ,
Abstract :
The levels of endogenous pig liver cells mitochondrial DNA oxidative base damage have been investigated using isotope dilution gas chromatography mass spectrometry (GC/MS). Higher levels of five measured bases were found in mtDNA in relation to nuclear DNA. We have also detected large differences in the modified base ratios of mitochondrial versus nuclear DNA. These ratios for the bases with promutagenic properties as 8OHGua and 5OHCyt are much lower then for other bases (5OHHyd, 5OHMeHyd, 5OHMeUra).
Keywords :
DNA damage , mitochondrial DNA , free radicals , oxidative damage
