Title of article
Separation of 100-Kilobase DNA Molecules in 10 Seconds
Author/Authors
Cox، Edward C. نويسنده , , Austin، Robert H. نويسنده , , Bakajin، Olgica نويسنده , , Duke، Thomas A. J. نويسنده , , Tegenfeldt، Jonas نويسنده , , Chou، Chia-Fu نويسنده , , Chan، Shirley S. نويسنده ,
Issue Information
دوهفته نامه با شماره پیاپی سال 2001
Pages
-6052
From page
6053
To page
0
Abstract
Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and gamma(48.5 kbp) DNAs could be resolved into two clearly separated bands in ~10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.
Keywords
High-performance liquid chromatography-mass spectrometry (LC-MS) , Solid-phase extraction (SPE) , red clover , Trifolium pratense , Isoflavone glycoside 6"-O-malonates , Isoflavone glycoside 6"-O-acetates
Journal title
Analytical Chemistry
Serial Year
2001
Journal title
Analytical Chemistry
Record number
51982
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