Title of article :
Oxidative stress and lipid mediators induced in alveolar macrophages by ultrafine particles
Author/Authors :
Ingrid Beck-Speier، نويسنده , , Niru Dayal، نويسنده , , Erwin Karg، نويسنده , , Konrad L. Maier، نويسنده , , Gabriele Schumann، نويسنده , , Holger Schulz، نويسنده , , Manuela Semmler-Behnke ، نويسنده , , Shinji Takenaka، نويسنده , , Kurt Stettmaier، نويسنده , , Wolf Bors، نويسنده , , Andy Ghio، نويسنده , , James M. Samet، نويسنده , , Joachim Heyder، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2005
Abstract :
In ambient aerosols, ultrafine particles (UFP) and their agglomerates are considered to be major factors contributing to adverse health effects. Reactivity of agglomerated UFP of elemental carbon (EC), Printex 90, Printex G, and diesel exhaust particles (DEP) was evaluated by the capacity of particles to oxidize methionine in a cell-free in vitro system for determination of their innate oxidative potential and by alveolar macrophages (AMs) to determine production of arachidonic acid (AA), including formation of prostaglandin E2 (PGE2), leukotriene B4 (LTB4), reactive oxygen species (ROS), and oxidative stress marker 8-isoprostane. EC exhibiting high oxidative potential induced generation of AA, PGE2, LTB4, and 8-isoprostane in canine and human AMs. Printex 90, Printex G, and DEP, showing low oxidative capacity, still induced formation of AA and PGE2, but not that of LTB4 or 8-isoprostane. Aging of EC lowered oxidative potential while still inducing production of AA and PGE2 but not that of LTB4 and 8-isoprostane. Cellular ROS production was stimulated by all particles independent of oxidative potential. Particle-induced formation of AA metabolites and ROS was dependent on mitogen-activated protein kinase kinase 1 activation of cytosolic phospholipase A2 (cPLA2) as shown by inhibitor studies. In conclusion, cPLA2, PGE2, and ROS formation was activated by all particle types, whereas LTB4 production and 8-isoprostane were strongly dependent on particlesʹ oxidative potential. Physical and chemical parameters of particle surface correlated with oxidative potential and stimulation of AM PGE2 and 8-isoprostane production.
Keywords :
Ultrafine particles , free radicals , 8-Isoprostane , alveolar macrophages , Lipid mediators , oxidative stress , phospholipase A2 , prostaglandin E2 , leukotriene B4
Journal title :
Free Radical Biology and Medicine
Journal title :
Free Radical Biology and Medicine