Title of article :
Effect of L-Carnitine on Mitochondrial Acyl CoA Esters in the Ischemic Dog Heart
Author/Authors :
Akira Kobayashi، نويسنده , , Shigeki Fujisawa، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1994
Pages :
10
From page :
499
To page :
508
Abstract :
Many studies have shown that L-carnitine has a positive effect on ischemic myocardium, probably by reducing accumulation of long-chain acyl coenzyme A (CoA) esters. Previous studies have involved whole-heart extracts and have not assessed changes of CoA ester levels in mitochondria, the site of translocase inhibition. To more precisely assess L-carnitine effects, we measured long-chain acyl CoA ester levels in cytosol and in mitochondria in the ischemic canine heart. Dogs were divided into four groups: a sham-operated control group; an untreated group; and high- and low-dose L-carnitine-treated groups (30 mg/kg and 100 mg/kg). After 60 min of ischemia, the heart was excised, and the cytosolic and mitochondrial fractions were isolated. CoA esters and the activity of carnitine palmitoylcarnitine transferase (CPT) I and II were measured in both compartments. Approximately 89% of cellular free CoA, 90% of cellular acetyl CoA, 97% of cellular shot-chain acyl CoA, and 92% of cellular long-chain acyl CoA were located in the mitochondrial space under the normal condition. Under the ischemic condition, mitochondrial free CoA was significantly decreased. Conversely, mitochondrial acetyl CoA and long-chain acyl CoA were significantly increased. Treatment with L-carnitine significantly decreased acetyl CoA and long-chain acyl CoA in the ischemic mitochondrial space in a dose-dependent manner. These results support the hypothesis that L-carnitine reduces accumulation of long-chain acyl CoA within the ischemic mitochondrial space and thereby improves mitochondrial function and adenine nucleotide translocation.
Keywords :
Carnitine , mitochondria , Long-chain acyl CoA , Ischemic myocardium , Cellular distribution
Journal title :
Journal of Molecular and Cellular Cardiology
Serial Year :
1994
Journal title :
Journal of Molecular and Cellular Cardiology
Record number :
525115
Link To Document :
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