Endothelin-1 contributes to ischemia/reperfusion injury in isolated rat heart—attenuation of ischemic injury by the endothelin-1 antagonists BQ123 and BQ610

. Endothelin-1 Contributes to Ischemia/Reperfusion Injury in Isolated Rat Heart—Attenuation of Ischemic Injury by the Endothelin-1 Antagonists BQ123 and BQ610. Journal of Molecular and Cellular Cardiology (1995) 27, 761–766. A potential detrimental role of endothelin-1 in myocardial ischemia/reperfusion injury was studied by use of the endothelin-1 antagonists BQ123 and BQ610. Isolated isovolumetric rat hearts were perfused at constant pressure. BQ123 (7 μg/min) and BQ610 (1.75 μg/min) did not alter mechanical Function or coronary flow and shifted dose-response curves for andothelin-1 significantly to the right. In rats subjected to 30 min of no-flow ischemia, the increase of left ventricular resting pressure was significantly delayed by BQ123 and BQ610 compared to control (BQ123: 20 ± 2* mmHg, BQ610:19 ± 2* mmHg, control: 44 ± 4 mmHg at 15 min of ischemia, respectively, *P