Preparation of 99mTcN(CBDTC)2 and its biodistribution in mice

In order to seek a new class of brain perfusion imaging agent containing [99mTcN]2+ core, bis(N-cyclobutyl dithiocarbamato) nitrido technetium-99m complex [99mTcN(CBDTC)2] (CBDTC: N-cyclobutyl dithiocarbamato) was synthesized through a simple and efficient method, which can be utilized for the preparation of a radiopharmaceutical through a lyophilized kit formulation. The radiochemical purity of the complex was over 90%, as measured by thin layer chromatography. No decomposition of the complex at room temperature was observed over a period of 6 h. Itʹs partition coefficient indicated that it is a good lipophilic complex. Biodistribution in mice showed that the complex was significantly retained in the brain. The brain uptake (ID %/g) was 3.61, 3.15 and 2.62 and the brain/blood ratio was 1.00, 1.44 and 1.30 at 5, 30 and 60 min post-injection, respectively. These results suggest that this compound could be a potential brain perfusion imaging agent.