Characterization and validation of the gamma-scintigraphic method for determining liquid holdup in foam

Characterization and validation protocols for quantifying liquid content in a protein foam by gamma-scintigraphy were developed. Spatial resolution, count resolution and volume resolution values were measured to determine the analytical limitations of the apparatus. Scintigraphic measures of volume, in both liquid and dispersed media, correlated well with physical measures. Ionic interaction between [99mTc]pertechnetate and bovine serum albumin was undetectable by gel permeation. These results confirm that gamma-scintigraphy can be used to quantify liquid holdup in protein foams of pharmaceutical interest.